Organic and Inorganic Nitrogen Uptake by Sarracenia Purpurea at Harvard Forest and Fort Albany ON 2007

doi:10.6073/pasta/2e456c7efe5ccd93d02b263845902baa Organic and Inorganic Nitrogen Uptake by Sarracenia Purpurea at Harvard Forest and Fort Albany ON 2007 Jim Demetrios Karagatzides Aaron Ellison https://orcid.org/0000-0003-4151-6081 Jessica Butler Researcher 2023 English

The nitrogen-limited carnivorous pitcher plant Sarracenia purpurea and its associated detritus-based food web is a model system for studying plant nutrient dynamics. We tested if S. purpurea can directly take up intact amino acids and compared uptake of organic and inorganic forms of nitrogen (N) across a gradient of N deposition. At sites in Canada and the United States, individual pitchers with complete or incomplete food webs were fed U-13C-15N-glycine, U-13C-15N-phenylalanine and 15NH415NO3 individually and in mixture. Plants took up intact amino acids. Acquisition of each N form provided in isolation exceeded uptake of the same form in mixture. At the high deposition site, uptake of 15N from amino acids was higher than uptake of 15N from inorganic nitrogen. At the low deposition site, uptake of 15N from all three forms of N were similar. Completeness of the associated food web had no effect on 15N uptake. By taking up intact amino acids, Sarracenia purpurea can short-circuit the inorganic N cycle, thus minimizing potential bottlenecks in N availability that result from the plant’s reliance for nitrogen mineralization on a seasonally reconstructed food web operating on infrequent and irregular prey capture.

acid rain amino acids carnivorous plants food webs nitrogen nitrogen-15 stable isotopes LTER controlled vocabulary inorganic nutrients LTER core area Harvard Forest HFR LTER USA HFR default

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Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf146 Tom Swamp Tract (Harvard Forest), Fort Albany (James Bay Ontario Canada). Coordinates based on WGS84 datum. -80.5 -72.2 +52.8 +42.5 2007 2007 genus Sarracenia species purpurea northern pitcher plant complete Information Manager Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu

Experimental design We used a 72-hour pulse-chase experiment with isotopically enriched amino acids as our organic nitrogen (ON) source and ammonium nitrate (15NH415NO3) as our inorganic nitrogen (IN) source to determine if pitcher plants can acquire ON directly and to compare ON and IN uptake under different conditions. At each site, 125 mature individuals were selected with at least 3 live (no sign of senescence) mature pitchers (firm and open). Five of these plants were not manipulated with 15N and were harvested at the end of the experiment to determine baseline 15N and 13C natural abundance. The remaining 120 plants were randomly assigned to one of six treatment groups: U-Gly (98 atom% U-13C-15N-glycine), U-Phe (98 atom% U-13C-15N-phenylalanine), I15N (98 atom% 15NH415NO3), U-Gly plus unlabeled phenylalanine and unlabeled NH4NO3 (hereafter U-Gly+), U-Phe plus unlabeled glycine and unlabeled NH4NO3 (hereafter U-Phe+), or I15N plus unlabeled glycine and phenylalanine (hereafter I15N+). Plants within treatment groups were randomly assigned to one of two harvests (3- or 72-hr) and one of two food webs treatments (with complete food webs or food webs without the macroinvertebrate larvae [midges Metriocnemus knabi + mosquitoes Wyeomyia smithii). Larvae of the sarcophagid fly Fletcherimyia fletcheri were occasionally found in the pitchers and were excluded from all experimental pitchers. There were N = 5 pitchers for each treatment at each site. Any liquid in the pitchers, along with the food web, was removed from all experimental pitchers the day before the pulse-chase experiment began; the liquid removed (pitcher “liquor”) was kept for the food web manipulations. Following food web removal in the field, pitchers were rinsed with distilled water to remove as much detritus and as many microbes as possible and the pitcher opening was blocked with a fine nylon mesh to limit subsequent entry of animals and prey. In the laboratory, all living midge and mosquito larvae were removed from liquid collected from each pitcher and kept alive overnight in a solution of pitcher liquor. The next day, the largest pitcher on each plant was fed with one of the 15N treatments. We fed each manipulated pitcher with a 0.8 mM 15N solution (2 ml for Fort Albany and 9 ml for the larger pitchers at Tom Swamp) and an equal amount of pitcher liquor, resulting in pitchers filled to approximately three-quarters of their volume. Thus, all experimental pitchers contained an enriched (15N) nutrient solution along with the microbial component of the food web (supplied in the pitcher liquor). Pitchers at Fort Albany were fed 0.022 mg N, whereas the larger pitchers at Tom Swamp were fed 0.101 mg N. When we added only single forms of N (i.e., the U-Gly, U-Phe, and I15N treatments), all N added to the pitchers was enriched in 15N. When we added three forms of N (the U-Gly+, U-Phe+, and I15N+ treatments), only one-third of the N added to each pitcher was enriched in 15N; the remaining two-thirds was comprised of equal amounts of the other two forms as unlabeled N. For the complete food web treatments, we put invertebrate larvae into the pitchers immediately after we added the 15N solution. We added two midge and two mosquito larvae in each pitcher in the complete food web treatment at Fort Albany and nine midge and nine mosquito larvae in each pitcher in the complete food web treatment at Tom Swamp (i.e., 1 midge + 1 mosquito larva per ml of pitcher liquor). Unfed (control) pitchers for which we measured natural abundance of 13C and 15N also had complete food webs (pitcher liquor + midge + mosquito larvae).

Harvest Target pitchers were cut-off 3 or 72 hr after feeding with a stainless steel razor blade that was rinsed in 50% ethanol between cuttings. Pitcher liquid was transferred to a sealed sterile plastic tube and the pitcher was placed in a zip-lock plastic bag. Both were stored in a cooler with cold packs and shipped immediately to the laboratory for processing. Pitchers were cut open longitudinally, washed thoroughly with tap water, then rinsed with 0.5 mM CaCl2 to remove any amino acids from the surface, and finally rinsed three times with distilled-deionized water and transferred to paper bags. Midge and mosquito larvae were removed from the pitcher liquid with an eye dropper, transferred through three sequential baths of distilled-deionized water and stored in new sterile vials. Because of the small mass of larvae in each pitcher, larvae from the five replicates of each harvest x treatment combination were pooled into one composite larval sample. Plant and invertebrate samples were then oven-dried at 65 deg C for 48 h and then weighed.

Isotopic analyses Each entire pitcher or composite larval sample was ground to a fine powder in a stainless steel capsule with a stainless steel ball using a Wig-L-Bug mixer (Bratt Technologies, LLC., East Orange, New Jersey, USA). A 4-mg subsample of plant tissue or a 1-mg subsample of larva was then placed into an 8 × 5 mm tin capsule (Elemental Microanalysis Mason, Ohio, USA) and combusted in a Costech ECS4010 Elemental Analyzer and DeltaPlus XP mass spectrometer at the University of New Hampshire to measure 13C/12C, %C, 15N/14N and %N concurrently.

Harvard Forest Long-Term Ecological Research Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf146-01-nitrogen.csv nitrogen hf146-01-nitrogen.csv 25875 0e42be5391783d7698d8744570f7b2d5 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p14/hf146/hf146-01-nitrogen.csv site site FA Fort Albany TS Tom Swamp treat treatment Ctrl Control Gly 98 atom% U-13C-15N-glycine Phe 98 atom% U-13C-15N-phenylalanine IN 98 atom% 15NH415NO3 GlyPlus labeled Gly plus unlabeled phenylalanine and unlabeled NH4NO3 PhePlus labeled Phe plus unlabeled glycine and unlabeled NH4NO3 INPlus labeled IN plus unlabeled glycine and phenylalanine Inv... as prefix: treatments and measurements on invertebrate community (Tom Swamp only) Free text (unrestricted) .* harv harvest, elapsed time since feeding 0 3 hr 1 72 hr fw presence or absence of invertebrate food web 0 removed 1 included dm plant dry mass milligram 1 natural NA missing value ll leaf length centimeter 0.1 real NA missing value dn15 δ15N (‰) dimensionless 0.01 real NA missing value dc13 δ13C (‰) dimensionless 0.01 real NA missing value n nitrogen concentration (%) dimensionless 0.01 real NA missing value c carbon concentration (%) dimensionless 0.01 real NA missing value n15 percent of total nitrogen in plant as newly acquired 15N dimensionless 0.1 real NA missing value n.rec percentage of nitrogen fed to plant recovered in the sample dimensionless 0.1 real NA missing value c.rec percentage of carbon fed to plant recovered in the sample dimensionless 0.1 real NA missing value n.atm.ex 15N in sample expressed as atom% excess dimensionless 0.01 real NA missing value n.mg.ex 15N in sample expressed as mg excess milligram 0.001 real NA missing value n.nmol.ex 15N in sample expressed as nmol excess nanomole 0.01 real NA missing value c.atm.ex 13C in sample expressed as atom% excess dimensionless 0.001 real NA missing value n15.dm nmol of 15N per mg of plant dry mass nanomole 0.0001 real NA missing value unh.no analysis number assigned to sample by the University of New Hampshire Stable Isotope Laboratory analysis number assigned to sample by the University of New Hampshire Stable Isotope Laboratory plant.no sample number assigned for laboratory work at Harvard Forest sample number assigned for laboratory work at Harvard Forest n.fed amount of 15N in mg fed to plant milligram 0.001 real NA missing value c.fed amount of 13C in mg fed to plant milligram 0.001 real NA missing value 262