uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/7f051b161a9e102c93a002430691a3fc Fern Perkins Howard Neufeld 2023 English

Atmospheric N has quadrupled due to anthropogenic inputs and is projected to double again by 2050. The lichen Umbilicaria mammulata relies primarily on atmospheric deposition for nutrients, including N. Much of this species’ geographic distribution coincides with the N deposition gradient in the eastern US. We were interested in the potential impacts of N deposition on this species under realistic field conditions. Lichens were collected at six sites, ranging from Maine (low deposition) to West Virginia (high deposition). Thalli were analyzed for %C, %N, C:N, specific thallus weight (a measure of thickness), chlorophyll content, and chlorophyll fluorescence. Using stepwise regression we identified the most predictive model(s) using N deposition, environmental variables, and climate to predict differences in lichen ecophysiology and biochemistry across the gradient. While N deposition was important in predicting some responses, the model that best predicted %N in lichen only included mean minimum temperature, mean annual precipitation, and substrate slope. In controlled experiments at Grandfather Mountain (Linville NC) lichens were fertilized in situ with N to mimic deposition projected for 2050. There were no significant differences between control and fertilized thalli for any response variables, but differences did exist among sites. The results of this study highlight the complexities associated with sampling along a gradient of one factor in a multivariate environment.

carbon chlorophyll lichens nitrogen deposition LTER controlled vocabulary disturbance human-environment LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf164 Simes Tract (Harvard Forest). Coordinates based on WGS84 datum. -72.212 -72.212 +42.469 +42.469 245 245 meter 2006 2006 genus Umbilicaria species mammulata rock tripe lichen complete Information Manager Harvard Forest

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(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

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Lichen thalli were collected from rock substrate using a putty knife. Thalli were collected in a manner so that their absence was not obvious (e.g. neighboring thalli were not collected). Chlorophyll was extracted in DMF.

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(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf164-01-nitrogen.csv nitrogen hf164-01-nitrogen.csv 456 aaadd7904c878908bce28f0655be257c 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p16/hf164/hf164-01-nitrogen.csv date date YYYY-MM code code code c percent carbon in lichen dimensionless 0.01 real NA missing value n percent nitrogen in lichen dimensionless 0.01 real NA missing value c.n ratio of carbon to nitrogen in lichen dimensionless 0.01 real NA missing value fv.fm variable divided by maximum chlorophyll fluorescence dimensionless 0.001 real NA missing value area area of the plastiquinone pool squareMillimeters 1 whole NA missing value stw lichen thickness millimeter 1 whole NA missing value chla.area ug of chlorophyll a per mm^2 of lichen microgramPerMillimeterSquared 0.001 real NA missing value chla.weight ug of chlorophyll a per mg of lichen microgramPerMilligram 0.001 real NA missing value chlb.area ug of chlorophyll b per mm^2 of lichen microgramPerMillimeterSquared 0.001 real NA missing value chlb.weight ug of chlorophyll b per mg of lichen microgramPerMilligram 0.001 real NA missing value chlab.area ug of chlorophyll per mm^2 of lichen microgramPerMillimeterSquared 0.001 real NA missing value chlab.weight ug of chlorophyll b per mg of lichen microgramPerMilligram 0.001 real NA missing value ph.chl ratio of phaeophytin to chlorophyll in lichen dimensionless 0.001 real NA missing value 4 community short-term measurement