uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/1bcdb8d01ce10c9029677d6a3dde84c3 Kristina Stinson Amy Mays Researcher 2023 English

Glossy buckthorn (Frangula alnus), an invasive shrub from Eurasia, colonizes both upland and mesic sites in New England, USA, reducing the growth and survival of native tree saplings and lowering species richness. Although a generalist, buckthorn thrives particularly well along river, pond, and wetland margins, which are traditional habitat for speckled alder (Alnus incana ssp. rugosa), a native nitrogen-fixing shrub. As buckthorn’s dense, monospecific growth is typical of allelopaths, we wondered whether it chemically suppresses the growth of alder and other indigenous shrubs. We thus propagated three native shrub species: Alnus incana ssp. rugosa, Viburnum dentatum and Spiraea latifolia, in invasive buckthorn and native dogwood (Cornus amomum) root and leaf mulch. After seven weeks, alder grown in buckthorn root demonstrated significantly smaller basal diameter than alder grown in buckthorn leaf or other mulches. Therefore, putative buckthorn allelopathy to alder likely occurs through root exudation instead of leaf litter effects. Meadowsweet grown in buckthorn mulch, in contrast, was taller, thicker, and had more numerous leaves than meadowsweet grown in native mulch. These species-specific effects point to allelopathy as a mechanism by which buckthorn changes the structure of native plant communities.

competition invasive species wetlands LTER controlled vocabulary populations LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf172 Harvard Forest Experimental Lath Houses. Coordinates based on WGS84 datum. +42.53 +42.53 -72.19 -72.19 330 330 meter 2008 2008 genus Frangula species alnus genus Alnus species incana ssp. rugosa genus Spiraea species latifolia complete Information Manager Harvard Forest

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We purchased all bioassay plants from New England Wetland Plants (NEWP), a restoration nursery in Amherst, Massachsetts. NEWP growers started alder from local seed in the spring of 2006, and propagated arrowwood and meadowsweet from cuttings made in Amherst in June of 2007. These plants, similar in size, overwintered for one season in Shutesbury, Massachusetts prior to their inclusion in this experiment. During experimental set-up, I inoculated each alder plant with 150 ml of aqueous-medium Frankia. Treatments: (a) Frangula alnus (glossy buckthorn) root (FR). (b) Frangula alnus leaf (FL). (c) Cornus amomum (silky dogwood) root (CR). (d) Cornus amomum leaf (CL). (e) no mulch (0). We gathered root and leaf tissue of both Frangula alnus and Cornus amomum in September 2007 from three different sites in the Pioneer Valley of Massachusetts: Wentworth Farm and Plum Brook Conservation Areas in Amherst and, in neighboring Shutesbury, at the corner of Buffam and North Valley Roads. I harvested material from three different plants at each site, which I then air-dried for a month and packed in sealed Ziploc bags for storage through the winter. In May 2008, Plant, Soil and Water Laboratory in Athens, Georgia analyzed the ratio of carbon to nitrogen present in each mulch type. In July 2008, I applied mulches to bioassay plants in 12% dry weight concentrations of mulch tissue to soil. Every two and a half weeks, until final data collection on 8/26/08, I measured bioassay plant treatment responses Soil: Sungro Sunshine Mix #2 with no fertilizer charge to avoid deactivating the N-fixing capacity of Frankia. Replications: 12.

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(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf172-01-frangula.csv Frangula hf172-01-frangula.csv 45855 b970f5507ae5c9510a7aa03c81344e93 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p17/hf172/hf172-01-frangula.csv date date YYYY-MM-DD 1 day plant.num randomly assigned identification tag randomly assigned identification tag bioassay.sp. bioassay species A Alnus incana S Spirea latifolia V Viburnum dentatum treatment.sp. treatment specis C Cornus amomum F Frangula alnus o control (no mulch) treatment.organ tretment organ L leaf R root o contro (no mulch) survival survival 1 yes NA bioassay plant dead height height of bioassay plant centimeter 0.1 real NA missing value basal.diam basal diameter of bioassay plant millimeter 0.1 real NA missing value leaf.num.live number of live leaves on bioassay plant number 1 natural NA missing value leaf.num.dead number of dead leaves on bioassay plant number 1 natural NA missing value branch.num.live number of living branches on bioassay plant number 1 natural NA missing value branch.num.dead number of dead branches on bioassay plant number 1 natural NA missing value flower.num number of flowers on bioassay plant number 1 whole NA missing value fruit.num number of fruits on bioassay plant number 1 whole NA missing value notes notes notes 900 invasive short-term measurement