uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/f76fa08775d5ad61280c6f35fbe43dbb Christopher Williams https://orcid.org/0000-0002-5047-0639 Myroslava Khomik https://orcid.org/0000-0002-0377-8833 Researcher 2023 English

Soil properties (C:N ratio, pH, soil moisture, bulk density, litter layer thickness) were measured at the clear cut site in December 2012. Measurements were taken inside the large PVC collars that were used to measure soil respiration at the sites during the 2012 growing season. The collars were removed for storage thereafter. 15 locations in total were sampled: 3 collars were in trenched plots, and 12 on untrenched soil. Soil properties were collected in order to help interpret spatial variability in the corresponding soil CO2 fluxes measured at the site.

carbon decomposition litter nitrogen ph soil moisture soil organic matter LTER controlled vocabulary primary production disturbance LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf227 Prospect Hill Tract (Harvard Forest). Coordinates based on WGS84 datum. -72.174 -72.174 +42.546 +42.546 403 403 meter 2012 2012 complete Information Manager Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu

Soil samples were collected on Dec 4th and Dec 5th, 2012. Both days were warm (about 10oC), but Dec 4th was foggy and sometimes light drizzle, while Dec 5th was part cloud. There was snow on the ground the week before.

Where possible 3 small cores were taken for bulk density (bd) analysis and gravimetric water content (GWC) and one larger core (about 10 cm thick) in the center for root analysis (this latter core was frozen in our lab at Clark University for later processing, for roots). Before coring the LFH horizon was removed/collected completely into a ziplock bag. Soil moisture was measured in three replicates outside of the collar using the Hydrosense moisture probe (%VWC), just like we did when we measured soil respiration during the growing season. Later we compared the GWC and VWC from the two different methods. Cores that were taken for bulk density analysis were also analyzed for CN ratio, by grinding the dried mineral soil to a fine powder, placing few mg into a small aluminum weighing boat and burning in a CN-analyzer that is in Torrey Labs (we prepared the samples and Manisha analyzed them for us). 15 collars were sampled, 3 of them were in trenched plots.

Soil pH, moisture (VWC and GWC), bulk density (with CN analysis on those mineral soil samples). pH was measured with a portable pH/moisture meter - KEL soil tester from the Harvard Forest supply room. VWC was measured without Hydrosense probe. Set volume cores were taken of the mineral soil just below the LFH-litter layer. The LFH-litter horizon inside the collar was collected (the height of that layer was noted and the diameter of the collars is known); a 15-20 cm core of roots was also taken. Sample processing was done in Torrey labs. Soils were dried in ovens at 105C in glass jars for 24-48hours, until constant weight. Leaves were dried in ovens, in coin envelopes, at 60oC for 24-48hours. All drying was done at Harvard Forest Labs. Dried soil samples were sieved to remove roots and any loose organic matter. All samples (leaves and soils) were ground individually using a mortar and pestle to a fine homogenous powder. Each ground sample was packed in triplicate and weighed on the Sartorius micro-balance (Sartorius) to 3 decimal places (once packed in the tin weighing boats): for leaves packed about 2mg; for soils packed about 10mg per replicate. The samples were analyzed on CHNOS Elemental vario micro-cube analyzer (Elementar Analysensystem, GmbH). The machine burns the sample and elemental ratio is determined as the area under the curve produced by the machine, from the signal. All is relative to internal standards (Acetanilide with known C:N % ratio); CN analysis completed by Manisha Pattel at Harvard Forest Lab. The output in the report that we should use is the % N and %C (these are % of mg weight that was put in - i.e. the final packet.weight)

Internal diameter (mm): rep1 = 41.32, rep2 = 41.35, rep3 = 41.31, average = 41.33, standard deviation = 0.02 Height (mm): rep1 = 30.18, rep2 = 30.32, rep3 = 30.24, average = 30.25, standard deviation = 0.07. Corer volume = 40572.16 mm3.

Harvard Forest

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(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf227-01-soil.csv soil characteristics hf227-01-soil.csv 1826 64aff2915bb1503cabb83511c2eae236 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p22/hf227/hf227-01-soil.csv collar collar identification. CC indicates untrenched, T indicates trenched. collar identification. CC indicates untrenched, T indicates trenched. NA missing value lfh.thickness thickness of LFH horizon centimeter 0.1 real NA missing value lfh.dry.weight weight of dried LFH-horizon inside the collar gram 0.01 real NA missing value lfh.c estimated C-content of LFH horizon - taken as 50% of dry weight gram 0.001 real NA missing value soil.water water content of mineral soil sample in grams gram 0.01 real NA missing value soil.ph pH of soil dimensionless 0.1 real NA missing value gwc.mean average % gravimetric water content of soil samples (g of water per g of dry soil) dimensionless 1 real NA missing value gwc.sd standard deviation of % GWC dimensionless 1 real NA missing value bd.mean average mineral soil bulk density gramsPerCubicCentimeter 0.01 real NA missing value bd.sd standard deviation of soil bulk density gramsPerCubicCentimeter 0.01 real NA missing value vwc.mean average volumetric water content around the collar, measured with the Hydrosense (%) dimensionless 1 real NA missing value vwc.sd standard deviation of %VWC dimensionless 1 real NA missing value soil.repl soil replicates used to calculate all of the above averages and standard deviations soil replicates used to calculate all of the above averages and standard deviations NA missing value soil.n.per average % soil nitrogen dimensionless 0.01 real NA missing value soil.c.per average % soil carbon dimensionless 0.01 real NA missing value soil.n average soil nitrogen content milligram 0.01 real NA missing value soil.c average soil carbon content milligram 0.01 real NA missing value cn.mean average carbon to nitrogen ratio, calculated from %N and %C dimensionless 0.01 real NA missing value cn.repl number of replicate samples used to calculate all of the C- and N- averages and standard deviations number 1 natural NA missing value soil.n.per.sd standard deviation % soil nitrogen dimensionless 0.01 real NA missing value soil.c.per.sd standard deviation % soil carbon dimensionless 0.01 real NA missing value soil.n.sd standard deviation soil nitrogen content milligram 0.01 real NA missing value soil.c.sd standard deviation soil carbon content milligram 0.01 real NA missing value cn.sd standard deviation carbon to nitrogen ratio, calculated from %N and %C dimensionless 0.01 real NA missing value 15 informatics atmosphere community short-term measurement modeling https://wordpress.clarku.edu/cwilliams/ https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf224 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf222