Leaf Area in the Clearcut Site at Harvard Forest 2010-2012

doi:10.6073/pasta/ba9fea7b3093c352625a5c2f928de039 Leaf Area in the Clearcut Site at Harvard Forest 2010-2012 Christopher Williams https://orcid.org/0000-0002-5047-0639 Myroslava Khomik https://orcid.org/0000-0002-0377-8833 Researcher Richard MacLean Researcher Melanie Vanderhoof Researcher 2023 English

This dataset contributed to an estimate of leaf area by species at the Prospect Hill clearcut regeneration site, used in a publication to attribute measured changes in gross primary productivity over time to either changes in species composition and associated traits versus changes in total leaf area. The file reports data on leaf area by species from destructive harvest in select plots. It also reports leaf area and leaf weight from destructive harvest of the foliage of select individual species to characterize the vertical distribution of foliage for those species. Corresponding measurements of total plant area recorded with a LAI-2000 before and after harvest of this foliage is reported as well, useful for inferring the leaf versus stem/branch portions of light interception as needed for improved estimates of LAI with the light-interception method. Lastly, below we report the result of an August 2012 site-level survey of plant area index measured with the LAI-2000 and converted to leaf area index.

canopy cover foliage leaf area index leaves plant physiology LTER controlled vocabulary primary production disturbance human-environment LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf228 Prospect Hill Tract (Harvard Forest). Coordinates based on WGS84 datum. -72.174 -72.174 +42.546 +42.546 403 403 meter 2010 2012 genus Acer species pensylvanicum striped maple species rubrum red maple genus Aralia species nudicaulis wild sarsaparilla genus Betula species alleghaniensis yellow birch species lenta black/sweet birch species papyrifera paper birch genus Carex species spp. sedge genus Comptonia species peregrina sweetfern genus Dennstaedtia species punctilobula hayscented fern genus Dryopteris species spp. woodfern genus Fagus species grandifolia American beech genus Maianthemum species canadense Canadian may-lily genus Oryzopsis species asperifolia rough-leaved rice grass genus Picea species abies Norway spruce species glauca white spruce genus Pinus species strobus white pine genus Polygonum species sp. knotweed genus Prunus species pensylvanica pin cherry species serotina black cherry genus Quercus species rubra red oak genus Rumex species acetosella field sorrel genus Rubus species allegheniensis Allegheny blackberry species idaeus American red raspberry species sp. dewberry genus Sambucus species pubens elderberry genus Treintalis species borealis starflower genus Tsuga species canadensis eastern hemlock genus Vaccinium species angustifolium lowbush blueberry complete Information Manager Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu

Study Site The study site occupies roughly a 200 m x 400 m area (8 ha) near the top of Prospect Hill, within the Harvard Forest Long Term Ecological Research Site.

Leaf Area by Species – Destructive Harvests Species-specific leaf area (projected, one-sided leaf area per ground area occupied by an individual plant of a particular species) was sampled for a select number of species by destructive sampling. Two to five representative individuals of each species were selected. To minimize the method’s impact, the number of individuals destructively sampled was based on the prevalence of the species within the study area. Individuals were chosen to represent the size spectrum observed along the vegetation transects. The aerial ground coverage of each individual was estimated using two tape meters placed perpendicular to each other and estimating the enclosed square, oval or triangular shape occupied by the individual. Each individual was destructively sampled by removing all green leaves from the individual. Total (one-sided) leaf area was determined by scanning the leaves in the lab, using a LI-3000 leaf area meter (Li-COR Biosciences, Lincoln, NB, USA). The scanned leaf area was divided by the ground area occupied by the individual plant, to produce a leaf area estimate (LA) for the species. This value was used to calculate species-specific leaf area index from the line-intercept vegetation cover.

Tree Destructive Harvest in 2012 In summer 2012, our group sampled a few key species, similar to the way it was done in 2010, but covering a larger clump/area. We found patches of the same species in designated areas (i.e. out of the tower's footprint) and measured the area occupied by the crown of the clump, as projected on the ground. We destructively harvested all of the leaves in the canopy, in 50 cm layers (where applicable), starting from the ground and up. Then we scanned all of the harvested leaves using the LI-3000 leaf area meter, available at Harvard Forest Torrey Lab. The leaves were then packed into paper bags and oven dried. The weight of each layer and overall was measured once dried. - all this done at HF at Torrey labs. The leaves were then brought back to Clark and stored in a box in a cool, dry place (our growth chamber room). Only a single cluster per species was harvested. Measurements within each cluster (two on each side of the ellipse - farthest apart - pointing the instrument inside the cluster and using the 45deg angle cap) were also performed using the LI-2000 before and after the harvest (dawn/dusk, time permitting). For blackberry and fern patches, the sampling patch was square in shape and measurements with the LI-2000 were taken from two sides. Also for blackberry and fern, the harvest was completed in a single layer and everything was harvested from bottom up (logistically that was the best approach for bb), so no stalks remained to measure the post-harvest LAI. The difference in LI-2000 measurements before the harvest and post-harvest give "true" LAI, since contribution of branches is excluded by the post-harvest measurement.

Notes on Harvest in 2010 Melanie Vanderhoof and REUs harvested numerous species at the site and estimated their leaf area per canopy area, by estimating the projected canopy area of an individual, then harvesting all leaves of that individual, measured the harvested projected one-sided leaf area of that individual, and finally divided the total projected one-sided leaf area by the projected canopy area (this we call LA). Leaf area was measured by spreading out flat, in a single layer in a box of a fixed area, all the harvested leaves - per individual. To get the LAI (leaf area per ground area), one needs to multiply these LA numbers by the % ground covered by that species' canopy - get from line intercept method (then get leaf area per ground area).

LAI-2000 Sampling In late August 2012, site-level LAI was also estimated with the LAI-2000 Plant Canopy Analyzer (LI-COR Biosciences, Inc.) optical plant area meter. Measurements were performed along one of the five line-intercept transects used for species abundance and composition surveys, and were made just below the canopy, along 8 points, at dusk. A 45-degree angle cap was used and data were corrected accordingly. Two additional measurements were performed before and after the transect survey, but in an adjacent open area free of shading. The difference between the mean open and mean transect value provided an estimate of plant area index at our site. Measured plant area index (PAI) was translated to leaf area index (LAI), by correcting for branches sampled during our destructive harvesting campaign (described above for estimation of species-level leaf area, involving select patches of trees: red maple – Acer rubrum (6.6 m2); pin cherry – Prunus pensylvanica (9.7 m2) and black cherry – Prunus serotina (11.1 m2)). During that sampling campaign, we took measurements with the LAI-2000 instrument before and after leaf harvest within each patch. The corresponding ratio of LAI to PAI in defoliated patches was used to correct the measured site-total PAI to provide just the portion related to leaves (i.e. LAI).

Data Files The two data files represent two distinct samplings. The first data file corresponds to a vertically-stratified destructive harvest of leaves on select species, as described above under “Tree Destructive Harvest in 2012”. The second data file contains the total leaf area for species that were sampled within a particular ground area that contained vegetation cover primarily composed of that species, as described above under “Leaf Area by Species – Destructive Harvests."

Harvard Forest Long-Term Ecological Research Harvard Forest

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(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf228-01-stratified.csv vertically stratified hf228-01-stratified.csv 841 d1a0bf407d8488b392ead5906f4ed920 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p22/hf228/hf228-01-stratified.csv year year of sampling YYYY 1 year NA missing value date date of sampling YYYY-MM-DD 1 day NA missing value sample sample identification sample identification NA missing value leaf.area scanned and corrected leaf area collected from the plant squareCentimeters 0.01 real NA missing value dry.weight dry weight of the sample leaves collected gram 0.000001 real NA missing value ground.area ground area occupied by the projected-shadow of the plant that was harvested squareCentimeters 1 real NA missing value species species sampled pc Prunus pensylvanica rm Acer rubrum bc Prunus serotina bb Rubus allegheniensis fern Dennstaedtia punctilobula or Dryopteris spp. NA missing value lai calculated leaf area index (leaf per ground) dimensionless 0.000000001 real NA missing value li2000.pre LI-2000 reading below canopy before the leaves were harvested dimensionless 0.001 real NA missing value li2000.post LI-2000 reading below canopy after the leaves were harvested dimensionless 0.001 real NA missing value level1.la total leaf area of leaves collected in the layer from 0-50cm height above the ground squareCentimeters 0.01 real NA missing value level2.la total leaf area of leaves collected in the layer from 0-50cm height above the ground squareCentimeters 0.01 real NA missing value level3.la total leaf area of leaves collected in the layer from 100-150cm height above the ground squareCentimeters 0.01 real NA missing value level4.la total leaf area of leaves collected in the layer from 150-200cm height above the ground squareCentimeters 0.01 real NA missing value level5.la total leaf area of leaves collected above 200cn height above the ground squareCentimeters 0.01 real NA missing value level1.wgt dry leaf weight of leaves collected in the layer from 0-50cm height above the ground gram 0.01 real NA missing value level2.wgt dry leaf weight of leaves collected in the layer from 50-100 cm height above the ground gram 0.00000001 real NA missing value level3.wgt dry leaf weight of leaves collected in the layer from 100-150cm height above the ground gram 0.0000001 real NA missing value level4.wgt dry leaf weight of leaves collected in the layer from 150-200cm height above the ground gram 0.0000001 real NA missing value level5.wgt dry leaf weight of leaves collected above 200cn height above the ground gram 0.0000001 real NA missing value 5 hf228-02-whole.csv whole plant hf228-02-whole.csv 3268 75ed3400e5714a96ea352c188ce53967 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p22/hf228/hf228-02-whole.csv year year of sampling YYYY 1 year NA missing value sample sample identification sample identification NA missing value leaf.area scanned and corrected leaf area collected from the plant squareCentimeters 0.01 real NA missing value dry.weight dry weight of the sample leaves collected gram 0.01 real NA missing value ground.area ground area occupied by the projected-shadow of the plant that was harvested squareCentimeters 0.1 real NA missing value species species sampled ws Aralia nudicaulis canadian Maianthemum canadense fern Dennstaedtia punctilobula or Dryopteris spp. kw Polygonum sp. rb Rubus idaeus bb Rubus allegheniensis sf Comptonia peregrina hf Dennstaedtia punctilobula wf Dryopteris spp. sedge Carex spp. rg Oryzopsis asperifolia sorrel Rumex acetosella starflower Treintalis borealis db Rubus sp. eb Sambucus pubens blue Vaccinium angustifolium sm Acer pensylvanicum rm Acer rubrum yb Betula lenta pb Betula papyrifera be Fagus grandifolia sp Picea abies or Picea glauca wp Pinus strobus pc Prunus pensylvanica bc Prunus serotina ro Quercus rubra eh Tsuga canadensis NA missing value lai calculated leaf area index dimensionless 0.001 real NA missing value 108