uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/e79db0a6ab676844dba0f3dcf9ef84ab Rosanne Healy Gregory Bonito Matthew Smith Faye Rosin Researcher 2023 English

There is evidence that ectomycorrhizal Pezizales prevail on roots at woodland edges or habitats where trees are well spaced, and where there is minimal understory vegetation, soil pH is relatively high, and bare soil is a feature. This observation was repeated in a recent study of mitospores produced by these fungi, where they were found most often at woodland edges, road sides, the middle of paths, and lawns. A key feature that distinguishes Basidiomycota from Ascomycota is that the dikaryon necessary for sexual reproduction is generally formed early after spore germination in the former, but not until fruitbody formation in the latter. Our hypothesis is that the mitospores produced by ectomycorrhizal Pezizales act as spermatia, and that these spores are more effectively produced and dispersed from bare soil than from areas with a thick organic layer. Here we tested our hypothesis by comparing the community of ectomycorrhizal root fungi on red oak trees in Harvard Forest and the Arnold Arboretum.

fungi mycorrhizae oak roots LTER controlled vocabulary primary production disturbance LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf273 Harvard Forest, Arnold Arboretum (Jamaica Plain MA). Coordinates based on WGS84 datum. -72.198 -71.189 +42.542 +42.508 52 420 meter 2013 2013 phylum Ascomycota phylum Basidiomycota genus Quercus species rubra northern red oak complete Information Manager Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu

(1) Roots were sampled from a 10 x 10 x 15 cm soil core from 24 red oak trees in each site. (2) DNA from sporocarps and from 32 root tips from at least three roots from each tree was extracted, and distinct morphotypes included. Cenococcum has distinctive morphology, and when observed on root tips was excluded from the study in order to focus on Pezizales vs Basidiomycota. (3) Extracts were amplified using Sigma Extract ‘n Amp with ITS1f /ITS4 primers. (4) Amplicons were sequenced using the same primers, and resulting sequences Blasted in NCBI to ascertain identity based on greater than 97% similarity of ITS. (5) A t-test was conducted to calculate significance of differences between the richness and abundance of Pezizales ectomycorrhizal root tips as compared to Basidiomycota in both sites.

Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf273-01-ectomycorrhiza.csv ectomycorrhiza hf273-01-ectomycorrhiza.csv 159368 89724357d9f209d7dc92ca276d4deaaf 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p27/hf273/hf273-01-ectomycorrhiza.csv date.harvest date ectomycorrhiza roots were harvested YYYY-MM-DD 1 day NA missing value site site in Harvard Forest site in Harvard Forest NA missing value tree.acc.num tree accession number tree accession number NA missing value host.seq host tree sequenced from the roots host tree sequenced from the roots NA missing value date.sort date roots were sorted YYYY-MM-DD 1 day NA missing value root.lat number of the root lateral number 1 natural NA missing value cluster number of the root clusters number 1 natural NA missing value morphotype short ectomycorrhiza root morphotype description short ectomycorrhiza root morphotype description NA missing value notes.core notes from the core sampling notes from the core sampling NA missing value notes.root notes on other root tips present notes on other root tips present NA missing value ext.tube.num extraction tube number for sequencing of root tips extraction tube number for sequencing of root tips NA missing value blast closest BLAST match of ectomycorrhiza sequence followed by closest sporocarp blast match closest BLAST match of ectomycorrhiza sequence followed by closest sporocarp blast match NA missing value lat latitude latitude NA missing value long longitude longitude NA missing value canopy other trees in the canopy besides red oak other trees in the canopy besides red oak NA missing value 800 biodiversity regional short-term measurement