uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/513b0fab962abd1a4ebdeebe02bfebbc Kristen DeAngelis https://orcid.org/0000-0002-5585-4551 Jerry Melillo Serita Frey https://orcid.org/0000-0002-9221-5919 Jeffrey Blanchard https://orcid.org/0000-0002-7310-9678 Researcher Linda van Diepen Researcher 2023 English

As Earth’s climate warms, soil carbon pools and the microbial communities that process them may change, altering the way in which carbon is recycled in soil. In this study, we used a combination of metagenomics and bacterial cultivation to evaluate the hypothesis that experimentally raising soil temperatures by 5°C for 5, 8, or 20 years increased the potential for temperate forest soil microbial communities to degrade carbohydrates. Warming decreased the proportion of carbohydrate-degrading genes in the organic horizon derived from eukaryotes and increased the fraction of genes in the mineral soil associated with Actinobacteria in all studies. Genes associated with carbohydrate degradation increased in the organic horizon after 5 years of warming but had decreased in the organic horizon after warming the soil continuously for 20 years. However, a greater proportion of the 295 bacteria from 6 phyla (10 classes, 14 orders, and 34 families) isolated from heated plots in the 20-year experiment were able to depolymerize cellulose and xylan than bacterial isolates from control soils. Together, these findings indicate that the enrichment of bacteria capable of degrading carbohydrates could be important for accelerated carbon cycling in a warmer world.

bacteria climate change community dynamics fungi microbes soil soil warming LTER controlled vocabulary populations LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf302 Prospect Hill Tract (Harvard Forest). Coordinates based on WGS84 datum. -72.18 -72.18 +42.54 +42.54 365 365 meter 2011 2011 complete Information Manager Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu

Complete methods are published in the associated paper. Briefly, soils were collected and immediately flash frozen to -80C. Frozen soils were extracted for DNA, and DNA used for sequencing analysis and quantitative PCR, as described in the published paper. Amplicon sequence data is available at GenBank under accession numbers SRP040706, BioProject ID PRJNA242868.

Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf302-01-warming-quant-PCR.csv warming quantitative PCR hf302-01-warming-quant-PCR.csv 5561 54f686f593103e85cdc82bedfa995829 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p30/hf302/hf302-01-warming-quant-PCR.csv loc location PH Prospect Hill Long-term Warming Study BW Barre Woods warming study SP Soil Warming by Nitrogen Experiment NA missing value temp heating treatment Control control or disturbance control plots Heated 5C heat treatment plots NA missing value m.o soil type Mineral 0-10 cm depth mineral soil horizon Organic organic soil NA missing value type microbial group targeted microbial group targeted NA missing value sample plot id number plot id number NA missing value copies copies of the genome as a measure of abundance; genome copies detected per gram of soil number 1 real NA missing value 128 plot community short-term measurement https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf005