uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/4b226f589f8075796b366a91bf7bdf4a Morgan Furze https://orcid.org/0000-0001-9690-6218 Andrew Richardson https://orcid.org/0000-0002-0148-6714 Researcher Brett Huggett https://orcid.org/0000-0003-3237-2741 Researcher Donald Aubrecht Researcher Claire Stolz Researcher Mariah Carbone https://orcid.org/0000-0002-7832-7009 Researcher Molly Wieringa Researcher Catherine Chamberlain Researcher 2023 English

We measured nonstructural carbohydrate (NSC) concentrations throughout the year in the branches, stemwood, and roots of five temperate tree species. These NSC concentrations were used in two ways. First, we scaled up concentrations to the whole-tree level using allometric equations and compared NSC storage between these five species to determine the size and seasonal fluctuation of whole-tree total NSC budgets as well as the contribution of individual organs. Second, for four of these species, we assessed the radial patterns and seasonality of NSC concentrations in the stemwood based on contrasting wood anatomy (ring-porous vs. diffuse-porous).

carbohydrates carbon physiology roots seasonality LTER controlled vocabulary primary production LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf308 Prospect Hill Tract (Harvard Forest). Coordinates based on WGS84 datum. -72.188 -72.188 +42.535 +42.535 350 350 meter 2014 2014 genus Acer species rubrum red maple genus Betula species papyrifera paper birch genus Fraxinus species americana white ash genus Pinus species strobus white pine genus Quercus species rubra red oak complete Information Manager Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu

We selected mature trees for this study belonging to the following species: red oak (Quercus rubra L., n=6), white pine (Pinus strobus L., n=6), red maple (Acer rubrum, n=6), paper birch (Betula papyrifera, n=3), and white ash (Fraxinus americana L., n=3). Red oak and white ash are ring-porous trees, while red maple and paper birch are diffuse-porous trees. White pine was excluded when assessing the radial patterns and seasonality of NSC concentrations in the stemwood based on wood anatomy.

Each month throughout 2014, a stemwood core to the pith was collected from each tree with a standard 4.3-mm increment borer. In addition to a monthly stemwood core, we collected sunlit branches from the top of the canopy. We collected coarse root cores in January, April, June, August, October, and December 2014.

To obtain a detailed understanding of NSC storage, we subdivided organs for NSC analysis. Subdividing organs also allowed us to assess the radial patterns of NSC concentrations within the stemwood. Stemwood cores collected monthly throughout 2014 were subdivided into segments from the cambium (designated as 0) to the pith: 0-1 cm, 1-2 cm, 2-3 cm, 3-4 cm, 4-8 cm, and 8 cm-pith. Root cores were subdivided as 0-1 cm and 1 cm-pith. Branches (multi-year, 3-5 y) were ground in their entirety. Dried and ground tissues were analyzed to determine sugar and starch concentrations

We estimated the dry wood biomass of each subdivided organ (branch, stemwood, and root) using allometric scaling theory. We then paired the sugar and starch concentrations for each sample with the estimate of that component’s woody biomass. This was done for each sample per tree, and then the amounts were summed to estimate sugar and starch budgets (kg) for each organ.

Using the NSC data for the stemwood, we assessed the radial distribution and seasonality of sugar and starch concentrations for ring-porous and diffuse-porous trees.

Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf308-01-nsc.csv NSC budgets hf308-01-nsc.csv 29660 6ad8c1e40e7ba348eeafe0f1e2b2bf9e 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p30/hf308/hf308-01-nsc.csv year year YYYY 1 year NA missing value month month month NA missing value species species name species name NA missing value tag tree tag tree tag NA missing value organ woody organ woody organ NA missing value sugar sugar budget in woody organ kilogram 0.1 real NA missing value starch starch budget in woody organ kilogram 0.1 real NA missing value 720 hf308-02-nsc-concentrations.csv NSC concentrations hf308-02-nsc-concentrations.csv 104390 9eaa1d423e79093f8e204bb19d9416f2 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p30/hf308/hf308-02-nsc-concentrations.csv year year YYYY 1 year NA missing value month month month NA missing value species species name species name NA missing value tag tree tag tree tag NA missing value organ woody organ woody organ NA missing value increment woody organ segment starting from inside the bark and moving to the pith for stem cores and root cores woody organ segment starting from inside the bark and moving to the pith for stem cores and root cores NA missing value sugar sugar concentration milligramPerGram 0.1 real NA missing value starch starch concentration milligramPerGram 0.1 real NA missing value total total concentration milligramPerGram 0.1 real NA missing value 2064 community short-term measurement milligramPerGram