uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/f1411aa8efc919f74dec36a5b4c69c45 Aaron Ellison https://orcid.org/0000-0003-4151-6081 Elizabeth Farnsworth 2023 English

All fieldwork was conducted from 3 to 13 August 2001. Elevation and location of each site was determined using a handheld Trimble GPS (Trimble Navigation, Sunnyvale, California, USA). Bedrock type was determined from field forms provided by the U.S. Bureau of Land Management and the U.S. Forest Service, published sources, and field observations. At each site, five 50-mL surface water samples were collected in sterile plastic tubes, frozen, and shipped frozen to our laboratory in Massachusetts for chemical analysis. There, water pH and calcium concentration were measured with an Orion model 290A portable pH/ISE meter and Orion pH and calcium electrodes. Nitrogen (both NO3-N and NH4-N) and phosphorus (PO4-P) concentrations were determined spectrophotometrically using standard methods.

At each site, we measured size and shape of the largest mature, fully expanded pitcher on 10–12 distinct plants (genets) haphazardly chosen from among a stand of 500–10 000 plants growing in full sun. The sample size was limited by federal collecting permits, and we were permitted to collect only leaves (pitchers), not whole plants. For each leaf, we used a flexible fabric tape to measure the height (± 1 mm) of the pitcher’s tube from ground level to the base of the hood; the circumference (± 1 mm) of the hood from its base, around the top, and down to the mouth; and the length and height (± 1 mm) of each half of the pitcher’s “winged appendage”. Dial calipers were used to measure the two diameters (± 0.1 mm) of the pitcher “mouth” and the diameter of the pitcher “tube” at its base, halfway up its length, and just below the pitcher “hood”.

After measuring the leaf, we determined maximum photosynthetic rate (Aarea: μmol CO2 m-2 s-1) using a LI-COR 6200 infrared gas analysis system with a custom-built 4-L chamber that enclosed the pitcher hood, winged appendage, and upper 20 cm of the pitcher tube. The chamber was constructed of Lexan and included two fans (LI-COR) to ensure adequate mixing of air. All measurements of Aarea were taken between 0930 and 1430 hours and photosynthetic photon flux density (PPFD) always exceeded 1000 mmol m-2 s-1. Chlorophyll contents of the pitcher tube, hood, and winged appendage were then assayed in the field using a CCM-200 chlorophyll content meter. The CCM-200 reports relative units (0–100), which we converted to actual units (mg chlorophyll [a + b]/ m2) after calibrating the device with direct measures of chlorophyll content using acetone extraction procedure (the conversion equation was mg chlorophyll/m2 = 391.8 + 16.78 × CCM units). The pitchers were then cut at the base, pressed in the field to prevent drying and loss of area, and returned within 24 h to our laboratory in Massachusetts where we used a LI-COR 3000 leaf area meter to determine the total area (±1 mm2) of the pitcher and the area of the portion of the pitcher that had been enclosed in the photosynthesis measurement chamber. The tube and hood were cut lengthwise and laid flat to get accurate measures of their area, and the area of the flat winged appendage was measured separately. We then dried the pitchers at 70 °C to constant mass, and weighed (± 0.001 g) the hood, winged appendage, and tube (sections inside and outside the photosynthesis chamber separately).

Each portion of the pitcher—hood, winged appendage, and tube—was ground in a Wiley mill prior to sending the samples to the University of Vermont Agricultural and Environmental Testing Laboratory where the C, N, and mineral content (P, Ca, Mg, K, Na, Al, Mn, Cu, Zn, Cr, Co, and Ni) of the leaves were determined. Carbon and N content of the finely ground, dried samples were determined using a Leeman Lab model 440 CHN elemental analyzer. Mineral content was determined on microwave-digested samples using a Perkin-Elmer Optima 3000 DV inductively-coupled plasma (ICP) atomic emission spectrometer.

Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf327-01-daca-site-info.csv site information hf327-01-daca-site-info.csv 657 504bdbc82797f5ef8fc181faf26bad24 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p32/hf327/hf327-01-daca-site-info.csv site site name site name NA missing value code site code site code NA missing value ownership ownership BLM Bureau of Land Management USFS United States Forest Service latitude latitude degree 0.00000001 real NA missing value longitude longitude degree 0.00000001 real NA missing value elevation elevation meter 1 natural NA missing value bedrock bedrock type bedrock type NA missing value wetland.type wetland type Boggy marshlike fen Stream streamside NA missing value ph pH of water samples (mean of n=2 replicates) dimensionless 0.001 real NA missing value no3 nitrate in water samples (mg/L mean of n = 2 replicates) milligramsPerLiter 0.00001 real NA missing value nh4 ammonium in water samples (mg/L mean of n = 2 replicates) milligramsPerLiter 0.00001 real NA missing value ca calcium in water samples (mg/L mean of n = 2 replicates) milligramsPerLiter 0.001 real NA missing value daca.adults number of adult Darlingtonia californica individuals (per m2; n = 25 1-m2 quadrats) numberPerMeterSquared 0.1 real NA missing value daca.juveniles number of juvenile Darlingtonia californica individuals (per m2; n = 25 1-m2 quadrats) numberPerMeterSquared 0.1 real NA missing value daca.flowering number of flowering Darlingtonia californica individuals (per m2; n = 25 1-m2 quadrats) numberPerMeterSquared 0.1 real NA missing value 5 hf327-02-daca-traits.csv traits hf327-02-daca-traits.csv 27366 e4ab5a84daf3ad163ca92b36e2385ba9 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p32/hf327/hf327-02-daca-traits.csv site site code; see hf327-01-daca-site-info.csv for site names and characteristics site code NA missing value plant plant number; individual replicates number 1 natural NA missing value photo area-based maximum photosynthetic rate (μmol CO2 m-2 s-1; mean of n = three 30-second measurements) micromolePerMeterSquaredPerSecond 0.000000001 real NA missing value cond area-based stomatal conductance (μmol CO2 m-2 s-1; mean of n = three 30-second measurements) micromolePerMeterSquaredPerSecond 0.000000001 real NA missing value cint intercellular concentration of CO2 (ppm; mean of n = three 30-second measurements) dimensionless 0.00000001 real NA missing value rs stomatal resistance (s cm-1; mean of n = three 30-second measurements) secondsPerCentimeter 0.000000001 real NA missing value cs stomatal conductance (cm s-1; mean of n = three 30-second measurements) centimetersPerSecond 0.000000001 real NA missing value wing.chl estimated chlorophyll concentration of the pitcher “winged appendage” gramsPerSquareMeter 0.000000001 real NA missing value tube.chl estimated chlorophyll concentration of the pitcher “tube” gramsPerSquareMeter 0.000000001 real NA missing value hood.chl estimated chlorophyll concentration of the pitcher “hood” gramsPerSquareMeter 0.000000001 real NA missing value hood.area surface area of the pitcher “hood” squareMeter 0.000001 real NA missing value wing.area surface area of the pitcher “winged appendage” squareMeter 0.000001 real NA missing value tube.area surface area of the pitcher “tube” squareMeter 0.000001 real NA missing value hood.mass oven-dried mass of the pitcher “hood” gram 0.01 real NA missing value wing.mass oven-dried mass of the pitcher “winged appendage” gram 0.01 real NA missing value tube.mass oven-dried mass of the pitcher “tube” gram 0.01 real NA missing value c.hood C content of the pitcher “hood” gramsPerGram 0.0001 real NA missing value h.hood H content of the pitcher “hood” gramsPerGram 0.0001 real NA missing value n.hood N content of the pitcher “hood” gramsPerGram 0.0001 real NA missing value c.tube C content of the pitcher “tube” gramsPerGram 0.0001 real NA missing value h.tube H content of the pitcher “tube” gramsPerGram 0.0001 real NA missing value n.tube N content of the pitcher “tube” gramsPerGram 0.0001 real NA missing value c.wing C content of the pitcher “winged appendage” gramsPerGram 0.0001 real NA missing value h.wing H content of the pitcher “winged appendage” gramsPerGram 0.0001 real NA missing value n.wing N content of the pitcher “winged appendage” gramsPerGram 0.0001 real NA missing value ca.hood Ca content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value mg.hood Mg content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value k.hood K content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value p.hood P content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value na.hood Na content of the pitcher “hood” gramsPerGram 0.000000001 real NA missing value al.hood Al content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value mn.hood Mn content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value cu.hood Cu content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value zn.hood Zn content of the pitcher “hood” gramsPerGram 0.000000001 real NA missing value cr.hood Cr content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value co.hood Co content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value ni.hood Ni content of the pitcher “hood” gramsPerGram 0.00000001 real NA missing value ca.tube Ca content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value mg.tube Mg content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value k.tube K content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value p.tube P content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value na.tube Na content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value al.tube Al content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value mn.tube Mn content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value cu.tube Cu content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value zn.tube Zn content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value cr.tube Cr content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value co.tube Co content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value ni.tube Ni content of the pitcher “tube” gramsPerGram 0.00000001 real NA missing value ca.wing Ca content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value mg.wing Mg content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value k.wing K content of the pitcher “winged appendage” gramsPerGram 0.000000001 real NA missing value p.wing P content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value na.wing Na content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value al.wing Al content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value mn.wing Mn content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value cu.wing Cu content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value zn.wing Zn content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value cr.wing Cr content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value co.wing Co content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value ni.wing Ni content of the pitcher “winged appendage” gramsPerGram 0.00000001 real NA missing value stemhgt height of the pitcher from ground to below the “hood" meter 0.001 real NA missing value hoodlgnth circumference of the hood from the top of the stemhgt measurement over the hood and down to the opening of the pitcher “mouth” meter 0.001 real NA missing value basediam diameter of the pitcher “tube” at its base meter 0.0001 real NA missing value middiam diameter of the pitcher “tube” at its midpoint meter 0.0001 real NA missing value topdiam diameter of the pitcher “tube” at its top, just below the pitcher “hood” meter 0.0001 real NA missing value mouthdiam diameter of the pitcher “mouth” meter 0.0001 real NA missing value wing1l length of one of the two “winged appendages” meter 0.001 real NA missing value wing1w width of one of the two “winged appendages” measured at its point of attachment to the pitcher “mouth” meter 0.001 real NA missing value wing2l width of the second of the two “winged appendages” measured at its point of attachment to the pitcher “mouth” meter 0.001 real NA missing value wing2w width of the second of the two “winged appendages” measured at its point of attachment to the pitcher “mouth” meter 0.001 real NA missing value spread tip-to-tip distance of the “winged appendage” meter 0.001 real NA missing value 56 community short-term measurement https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf096 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf098 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf109 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf146 secondsPerCentimeter