uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/eab86a290cd2c61f3db9c8b14ebcc323 Aaron Ellison https://orcid.org/0000-0003-4151-6081 Nicholas Gotelli https://orcid.org/0000-0002-5409-7456 Amy Wakefield Researcher Sarah Wittman Researcher 2023 English

In May 2002 we haphazardly chose 200 nonflowering adult plants (rosettes ≥10 cm) in the open area of the bog. A distance of at least 50 cm separated all experimental plants. Each plant was randomly assigned to one of eight treatment levels (n = 25 plants per treatment), corresponding to 0-7 frozen houseflies (mean dry mass = 1 mg; 103 mg N/g; 7.6 mg P/g; 9.2 mg K/g; 1.7 mg Ca/g; 1.4 mg Mg/g) fed twice weekly to each pitcher throughout the summer. Flies were added only to pitchers produced during the 2002 growing season; prey addition began when the first new pitchers were produced (24 June 2002) and continued until 12 September 2002. We added distilled water as necessary to maintain pitcher fluid at half of the leaf volume (5–10 mL). Pitchers on each plant were numbered sequentially in order of opening date (Leaf I, Leaf II, Leaf III, etc.) and each leaf was fed until they were harvested for analysis.

At approximately two-week intervals (10 and 29 July, 13 August, 2 and 15 September), all fed pitchers were harvested from each of five randomly chosen plants from each treatment group. Immediately before each harvest, dissolved oxygen content of the pitcher fluid (milligrams per liter and percentage saturation) of each leaf designated for that harvest was measured in situ using a YSI-58 dissolved oxygen meter (YSI instruments, Yellow Springs, Ohio, USA). Each pitcher was then cut off at the base; pitcher fluid was poured into sterile plastic tubes (50-mL centrifuge tubes) for subsequent analysis and the pitcher itself placed into a Ziploc plastic bag. All harvested material was kept cool and returned to the laboratory at the University of Vermont for processing within 4 h. In the laboratory, the pitcher fluid was filtered using a Millipore Sterifil Aseptic system. The pH of the filtrate was measured with an Orion Model 290A portable pH/ISE meter, and nitrogen (both NO3-N and NH4-N) and phosphorus (PO4-P) concentrations were determined spectrophotometrically using standard methods.

The length, mouth diameter, and maximum width (±1 mm) of each harvested pitcher or phyllode was measured, along with the width of its wing (keel). All leaves were dried at 70 °C for 3–5 days, and individually weighed and ground for nutrient analysis at the University of Vermont Agricultural and Environmental Testing Laboratory (Burlington, Vermont, USA).

On 15 August 2002 we measured maximum photosynthetic rates of the Leaf I on two randomly selected plants from each treatment level. These plants were all harvested at the fourth harvest (2 September). Photosynthesis measurements were made in full sun, between 09:00 and 14:00 hours, using a LI-COR 6200 photosynthesis system and custom built 4-L chamber. During all measurements, ambient solar radiation exceeded the saturating photosynthetic photon flux density of Sarracenia (PPFD > 800 mmol·m-2·s-1).

Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf328-01-sapu-prey-additions-2002.csv prey additions hf328-01-sapu-prey-additions-2002.csv 20686 9345cff725be9d15af6cb48a154eb4ff 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p32/hf328/hf328-01-sapu-prey-additions-2002.csv plantnum individual plant number individual plant number NA missing value fliesperwk number of flies added each week number 1 whole NA missing value harvest harvest date 1 10 July 2002 2 29 July 2002 3 13 August 2002 4 2 September 2002 5 15 September 2002 NA missing value do.mg.l dissolved oxygen concentration milligramsPerLiter 0.01 real NA missing value do.pct dissolved oxygen (percent saturation) dimensionless 0.1 real NA missing value halfvol half of the total volume of the pitcher milliliter 0.1 real NA missing value lflen leaf length, measured from the base of the pitcher to the top of the hood millimeter 0.01 real NA missing value lfwid maximum leaf width, measured from the midrib and including the keel millimeter 0.01 real NA missing value keelwid maximum width of the keel, from its outer edge to the edge of the pitcher tube millimeter 0.01 real NA missing value diam1 larger diameter of the elliptical pitcher mouth millimeter 0.01 real NA missing value diam2 smaller diameter of the elliptical pitcher mouth, measured perpendicular to diam1 millimeter 0.01 real NA missing value leafarea leaf area of the flattened pitcher squareCentimeters 0.01 real NA missing value leafmass dry mass of the pitcher gram 0.01 real NA missing value ph pH of the pitcher fluid dimensionless 0.01 real NA missing value no3 concentration of nitrate-N in the pitcher fluid milligramsPerLiter 0.01 real NA missing value nh4 concentration of ammonium-N in the pitcher fluid milligramsPerLiter 0.01 real NA missing value po4 concentration of phosphate-P in the pitcher fluid milligramsPerLiter 0.01 real NA missing value pct.n concentration of nitrogen in dried leaf tissue (percent) dimensionless 0.01 real NA missing value ca.ppm concentration of calcium in dried leaf tissue (ppm) dimensionless 0.1 real NA missing value p.ppm concentration of total phosphorus in dried leaf tissue (ppm) dimensionless 0.1 real NA missing value k.ppm concentration of potassium in dried leaf tissue (ppm) dimensionless 0.1 real NA missing value mg.ppm concentration of magnesium in dried leaf tissue (ppm) dimensionless 0.1 real NA missing value 197 community short-term measurement https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf096 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf098 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf109 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf146