uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/cb75dacd8dfbde1ae75ebb690cb42899 Aaron Ellison https://orcid.org/0000-0003-4151-6081 Nicholas Gotelli https://orcid.org/0000-0002-5409-7456 2023 English

In May 1998, we randomly selected 90 adult (rosette diameter ≥10 cm) S. purpurea plants at Hawley Bog, Massachusetts. We randomly assigned each of these plants to one of nine different nutrient treatments: two control treatments (distilled H2O or 10% concentration of micronutrients only from Hoagland’s solution); two N treatments (0.1 mg or 1.0 mg NH4-N/liter as NH4Cl); two phosphorus (P) treatments (0.025 mg or 0.25 mg PO4-P/liter as NaH2PO4); and three treatments in which we altered the N:P ratio. All N and P treatments also received micronutrients as 10% Hoagland’s solution. Every two weeks during the growing seasons (June 1 to September 30) of 1998, 1999, and 2000, 5 ml of the assigned nutrient solution was added directly to each open pitcher on each plant. pH of pitcher water in the experimental plants during the growing season remained within the range (3.5–5.5) observed in control pitchers. After each nutrient addition, leaves were plugged with glass wool to prevent colonization by common pitcher inhabitants and capture of prey. All leaves were measured in mid-September every year, before senescence. Pitcher height was measured with a flexible vinyl tape (±1 mm), and mouth diameter, tube width, and keel width were measured with calipers (±0.1 mm). Data: hf330-01-amax-morphology.csv In September 1998 and 1999 after measurements, the largest leaf on each plant was clipped, dried at 70 °C for 3–5 days, and individually weighed and ground for nutrient analysis at the University of Vermont Agricultural and Environmental Testing Laboratory. Carbon and N content of the finely ground, dried samples were determined using a Leeman Lab model 440 CHN elemental analyzer. Mineral content was determined on microwave-digested samples using a Perkin-Elmer Optima 3000 DV inductively-coupled plasma (ICP) atomic emission spectrometer. Data: hf330-02-leaf-stoichiometry.csv Photosynthetic rates (μmol of CO2 m-2 s-1) of the largest leaf on all surviving plants (n = 73) in the nutrient addition experiment were measured between 20 June and 3 July 2000, by using a Li-Cor Li-6200 photosynthesis system and custom-built 4-liter chamber. All measurements were made between 0900 and 1400 h, under ambient solar radiation that exceeded the light saturation point for S. purpurea (photosynthetic photon flux density >800 μmol m-2 s-1). Data: hf330-01-amax-morphology.csv

We conducted a second experiment in which we established experimental plots that contained S. purpurea and sprayed each plot with solutions of NH4NO3. Nine 2 × 2 m plots, each with 1–3 S. purpurea individuals, were established at Molly Bog, Vermont. Each plot was randomly assigned in a regression design to a different treatment: 0, 0.01, 0.025, 0.05, 0.1, 0.25, 0.5, 1.0, or 2.5 g N/m2 to be added over the growing season. We applied N as NH4NO3 in solution by using a backpack sprayer every 3 weeks from May 8 to September 11, 2000. P and K also were added to each plot as KH2PO4 to produce an application of 5 and 6.3 g/m2, respectively, over the growing season. In mid-September 2000, we measured the morphology of the largest S. purpurea leaf on each plant. Data: hf330-03-simulated-deposition.csv

We surveyed 26 bogs across Massachusetts and Vermont during the summer of 2000 (see dataset HF147-07 for geographic information about each site). At each bog, we measured the largest leaf of 25 randomly selected S. purpurea individuals and collected 5 samples of pore water by pressing a 50-ml sterile centrifuge tube into the Sphagnum mat. Tubes filled with water within 10 s. Concentration of NH4 and NO3 in pore water was determined by using salicylate and cadmium reduction spectrophotometry, respectively. In general, NO3 was below detection levels of instruments. Data: hf330-04-indicator-morphology.csv, hf330-05-indicator-chemistry.csv

Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. hf330-01-amax-morphology.csv amax morphology hf330-01-amax-morphology.csv 12927 6ec511b62d263b72bf1471d2a3fe1606 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p33/hf330/hf330-01-amax-morphology.csv date date YYYY-MM-DD 1 day NA missing value plantnum individual plant number individual plant number NA missing value stage stage A adult NA missing value treat treatment type con control dH2O distilled water only High-N high N High-P high P Low-N low N Low-P low P Micros Hoagland’s solution of micronutrients only N:P(1) N:P(1) N:P(2) N:P(2) N:P(3) N:P(3) NA missing value leaflen pitcher length, measured from the base of the pitcher to the top of the hood centimeter 0.1 real NA missing value leafwid maximum pitcher width, measured from the midrib and including the keel centimeter 0.1 real NA missing value keelwid maximum width of the keel, from its outer edge to the edge of the pitcher tube centimeter 0.1 real NA missing value mouthdiam larger diameter of the elliptical pitcher mouth centimeter 0.01 real NA missing value area leaf area of the flattened pitcher squareCentimeters 0.1 real NA missing value par photosynthetically active radiation (PPFD in μmol m-2 s-1) micromolePerMeterSquaredPerSecond 0.1 real NA missing value pmax maximum area-based photosynthetic rate (μmol of CO2 m-2 s-1) micromolePerMeterSquaredPerSecond 0.01 real NA missing value conductance area-based stomatal conductance (μmol of CO2 m-2 s-1) micromolePerMeterSquaredPerSecond 0.01 real NA missing value 214 hf330-02-leaf-stoichiometry.csv leaf stoichiometry hf330-02-leaf-stoichiometry.csv 21408 414b8ce4898bf93a79b9cb3b829fd24a 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p33/hf330/hf330-02-leaf-stoichiometry.csv year year (1998 or 1999) YYYY 1 year NA missing value plantnum individual plant number individual plant number NA missing value stage stage A adult J juvenile NA missing value treat treatment con control dH2O distilled water only High-N high N High-P high P Low-N low N Low-P low P Micros Hoagland’s solution of micronutrients only N:P(1) N:P(1) N:P(2) N:P(2) N:P(3) N:P(3) NA missing value mass mass of tissue sample used for tissue nutrient analysis kilogram 0.001 real NA missing value pct.c carbon concentration in leaf sample (percent) dimensionless 0.01 real NA missing value pct.h hydrogen concentration in leaf sample (percent) dimensionless 0.01 real NA missing value pct.n nitrogen concentration in leaf sample (percent) dimensionless 0.01 real NA missing value ca calcium concentration in leaf sample milligramPerKilogram 0.01 real NA missing value p phosphorus concentration in leaf sample milligramPerKilogram 0.01 real NA missing value mg magnesium concentration in leaf sample milligramPerKilogram 0.01 real NA missing value k potassium concentration in leaf sample milligramPerKilogram 0.01 real NA missing value 340 hf330-03-simulated-deposition.csv simulated deposition hf330-03-simulated-deposition.csv 2348 cda48b2ac0bdc18ba0e036e2b701f6ee 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p33/hf330/hf330-03-simulated-deposition.csv plot plot number plot number NA missing value n nitrogen added gramsPerSquareMeter 0.01 real NA missing value plant plant number plant number NA missing value leaf leaf number leaf number NA missing value leaflen leaf length, measured from the base of the pitcher to the top of the hood centimeter 0.1 real NA missing value liplen thickness of the pitcher lip millimeter 0.1 real NA missing value leafwid maximum leaf width, measured from the midrib and including the keel millimeter 0.1 real NA missing value keelwid maximum width of the keel, from its outer edge to the edge of the pitcher tube millimeter 0.1 real NA missing value diam1 larger diameter of the elliptical pitcher mouth millimeter 0.1 real NA missing value diam2 smaller diameter of the elliptical pitcher mouth, measured perpendicular to diam1 millimeter 0.1 real NA missing value 67 hf330-04-indicator-morphology.csv indicator morphology hf330-04-indicator-morphology.csv 33590 27f7276d08975572ab98e4828c989860 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p33/hf330/hf330-04-indicator-morphology.csv year year (2000) YYYY 1 year NA missing value state state MA Massachusetts VT Vermont NA missing value code site name code. See related link "Ant Distribution and Abundance in New England since 1990", hf147-07-ant-sites-1999-2000.csv, for site names, codes, and geographic information site name code NA missing value quadrat sampled quadrat number sampled quadrat number NA missing value leaflen leaf length, measured from the base of the pitcher to the top of the hood centimeter 0.001 real NA missing value diam larger diameter of the elliptical pitcher mouth millimeter 0.001 real NA missing value leafwid maximum leaf width, measured from the midrib and including the keel millimeter 0.001 real NA missing value keelwid maximum width of the keel, from its outer edge to the edge of the pitcher tube millimeter 0.001 real NA missing value rosette.diam maximum diameter of the rosette centimeter 0.1 real NA missing value pitchers number of pitchers number 1 whole NA missing value phyllodes number of phyllodes number 1 whole NA missing value repro reproductive status BUDAB aborted bud FL flower FLAB flower aborted FLHERB flower eaten by herbivore FR mature fruit FRHERB fruit eaten by herbivore NONE no evidence of reproduction 667 hf330-05-indicator-chemistry.csv indicator chemistry hf330-05-indicator-chemistry.csv 2469 2f172ea7a72cbcd7f15013e79e5be36b 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p33/hf330/hf330-05-indicator-chemistry.csv code site name code. See related link "Ant Distribution and Abundance in New England since 1990", hf147-07-ant-sites-1999-2000.csv, for site names, codes, and geographic information site name code NA missing value sample water sample number (1-3) water sample number (1-3) NA missing value ph pH of the water sample dimensionless 0.01 real NA missing value nh4 concentration of ammonium in water sample milligramsPerLiter 0.01 real NA missing value no3 concentration of nitrate in water sample milligramsPerLiter 0.01 real NA missing value ph4 concentration of phosphate in water sample milligramsPerLiter 0.01 real NA missing value ca concentration of calcium in water sample milligramsPerLiter 0.01 real NA missing value 80 community regional short-term measurement https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf096 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf098 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf109 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf146 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf147 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf202 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf205 micromolePerMeterSquaredPerSecond milligramPerKilogram