uid=HFR,o=lter,dc=ecoinformatics,dc=org all public read doi:10.6073/pasta/d0bc57386a32da55690eba86efbbad69 Primrose Boynton https://orcid.org/0000-0003-0720-8966 Samer El-Khatib https://orcid.org/0000-0003-1531-3002 Researcher Rosie Franklin Researcher Christopher Klein Researcher 2024 English

Fungi and bacteria are common members of the microbiomes of carnivorous plants. In the pitcher plant Sarracenia purpurea these microorganisms enter carnivorous pitchers shortly after pitchers develop, and may be relevant for pitcher functioning. We are sampling pitchers repeatedly over several years to better understand the culturable diversity in this habitat, with a focus on the common pitcher yeast Candida pseudoglaebosa and fungi that have the potential to interact with it. C. pseudoglaebosa dominates pitcher plants by arriving early in pitchers, and we are interested in how this yeast’s populations change over time and in response to other pitcher microorganisms. Between 2021 and 2023, we collected pitcher water from Tom Swamp in Harvard Forest and cultured 118 yeast and bacteria colonies from this water. We have found C. pseudoglaebosa and some other yeasts (Papiliotrema, Rhodotorula, and Sporidiobolus); microbial identification is ongoing. We are planning to investigate changes in C. pseudoglaebosa genetic diversity and changes in C. pseudoglaebosa interactions with other pitcher microorganisms over time.

bogs carnivorous plants community composition evolution fungi microbes LTER controlled vocabulary populations LTER core area Harvard Forest HFR LTER USA HFR default

This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

Creative Commons Zero v1.0 Universal https://spdx.org/licenses/CC0-1.0.html CC0-1.0 https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf445 Tom Swamp Tract (Harvard Forest). Coordinates based on WGS84 datum. -72.2 -72.2 +42.5 +42.5 240 240 meter 2021 2023 genus Candida species pseudoglaebosa genus Sarracenia species purpurea ongoing Information Manager Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 hf-im@lists.fas.harvard.edu Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu

We visited Tom Swamp in the summers of 2021, 2022, and 2023 to collect microbial cultures from S. purpurea pitchers. Pitchers were chosen haphazardly, and approximately 0.5 ml of liquid was removed from each pitcher with a sterile pipette and transported to the laboratory. We then diluted samples with sterile water and spread colonies onto pitcher plant microbe isolation medium (0.5% yeast extract and 2% agar). Antibiotics (50 µg/ml ampicillin, streptomycin, and penicillin) were added to the pitcher plant microbe isolation medium in 2021 but not 2023. After approximately one week, isolated colonies were picked and transferred to fresh medium without antibiotics; cultures were stored long-term in 20% glycerol at -80 °C. Yeast colonies were identified by sequencing the Internal Transcribed Spacer (ITS) region of ribosomal RNA-coding DNA using the ITS1/ITS4 primer pair.

Harvard Forest

324 North Main Street Petersham MA 01366 USA

(978) 724-3302 (978) 724-3595 https://harvardforest.fas.harvard.edu https://ror.org/059cpzx98 pointOfContact The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. Other funding: Wheaton College Faculty-Student Provost Research fund. hf445-01-microbial-sampling.csv microbial sampling data hf445-01-microbial-sampling.csv 992 64e48b0e497962fe7d6ce7d72856cdb8 1 \r\n column , https://harvardforest.fas.harvard.edu/data/p44/hf445/hf445-01-microbial-sampling.csv sampling_date date of sample collection from pitchers YYYY-MM-DD 1 sampling_year year of sample collection from pitchers YYYY 1 sampling_month month of sample collection from pitchers month sampling_day day of sample collection from pitchers nominalDay 1 integer NA missing value plant identity of each plant identifier pitcher identity of each pitcher identifier colonies number of colonies isolated from each sample dimensionless 1 integer NA missing value colonies_identified number of colonies from each sample for which the Internal Transcribed Spacer (ITS) region of ribosomal RNA-coding DNA was sequenced dimensionless 1 integer NA missing value taxa_identified taxa identified using ITS sequences from each sample identifier 17 biodiversity short-term measurement