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Harvard Forest Data Archive

HF441

Fungal Diversity in Sarracenia Purpurea Pitchers at Harvard Forest 2009-2010

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Data

Overview

  • Lead: Primrose Boynton, Anne Pringle
  • Investigators: Celeste Peterson
  • Contact: Information Manager
  • Start date: 2009
  • End date: 2010
  • Status: complete
  • Location: Tom Swamp Tract (Harvard Forest)
  • Latitude: +42.5 degrees
  • Longitude: -72.2 degrees
  • Elevation: 240 meter
  • Datum: WGS84
  • Taxa: Sarracenia purpurea, Candida pseudoglaebosa, Rhodotorula babjevae, Moesziomyces aphidis
  • Release date: 2023
  • Language: English
  • EML file: knb-lter-hfr.441.2
  • DOI: digital object identifier
  • EDI: data package
  • DataONE: data package
  • Related links:
  • Study type: short-term measurement
  • Research topic: biodiversity studies; physiological ecology, population dynamics and species interactions
  • LTER core area: population studies
  • Keywords: carnivorous plants, dispersal, fungi, microbes, succession
  • Abstract:

    The carnivorous pitcher plant Sarracenia purpurea is widely distributed in the United States and Canada, and is host to a variety of symbiotic organisms, including symbiotic fungi. Culturing of S. purpurea pitcher contents in its native range uncovers diverse single-celled (yeast) communities; these yeast communities are dominated by the ascomycete yeast Candida pseudoglaebosa. We set out to understand how fungal diversity in S. purpurea pitchers changes over space and time, and how C. pseudoglaebosa might influence this diversity.

    In the summer of 2009, we assayed S. purpurea pitcher water fungal succession in Tom Swamp in Harvard Forest. We sequenced fungal DNA barcodes from 43 pitchers at different times throughout the growing season, and found that C. pseudoglaebosa has a strong impact on fungal diversity. It generally appears in pitchers early in succession, and once it arrives, it often becomes dominant quickly and decreases community evenness. We also identified two other culturable yeasts, Rhodotorula babjevae and Moesziomyces aphidis, which are common but not dominant in pitchers. In laboratory experiments, C. pseudoglaebosa outcompetes these two yeasts, but only if it is inoculated in large numbers, suggesting that C. pseudoglaebosa’s dominance is a consequence of early arrival during pitcher succession.

    The following summer (2010), we collected water from pitchers at five distant sites in the United States and Canada, and assayed fungal community diversity and C. pseudoglaebosa genetic diversity. We sampled pitcher plants from Tom Swamp in Harvard Forest, plus four other sites in British Columbia, Newfoundland, Georgia, and Florida (Floridian plants were Sarracenia rosea, a close relative of S. purpurea). Fungal communities tended to be structured geographically, with close communities resembling each other more than distant communities. In contrast, C. pseudoglaebosa exhibited three populations: one well-mixed population including isolates from Harvard Forest, Georgia, and Newfoundland (East Coast), one from Florida, and one from British Columbia. Taken together, these results suggest that C. pseudoglaebosa dispersal is not limited along the East Coast, but that there may be other ecological factors preventing mixing between Florida and the East Coast population.

  • Methods:

    Pitcher identification and sample collection

    In the summer of 2009, we labeled 43 unopened S. purpurea pitchers with plastic ties and tracked their opening dates. We then aseptically collected about 0.25 ml of water from each pitcher using a sterile pipette 4 days, 6-9 days (approximately one week), 34-42 days (approximately one month), and 66-74 days (approximately two months) after the pitcher had opened. Samples were mixed with 0.25 ml of buffer and frozen before DNA extraction and fungal identification. One isolate each of C. pseudoglaebosa, R. babjevae, and M. aphidis were haphazardly isolated from Tom Swamp and Belchertown, MA before 2009 by spreading pitcher water onto growth medium with antibiotics and subsequently subculturing yeast-like colonies.

    In the summer of 2010, we located 34 S. purpurea or S. rosea pitchers in Parker Lake Ecological Reserve, BC, Canada; La Manche Provincial Park, NL, Canada; Harvard Forest, MA, USA; Tattnall County, GA, USA; and Applachicola National Forest, FL, USA. We collected water from pitchers of varying ages from each plant, mixing approximately 0.25 ml of pitcher liquid with 0.25 ml of buffer and freezing for DNA extraction and fungal identification. All samples from the same plant were pooled to get a mixed-age sample for each plant. We also spread liquid from these pitchers onto growth medium with antibiotics to isolate C. pseudoglaebosa for genotyping. Yeast colonies that morphologically resembled C. pseudoglaebosa were identified using sequences of their Internal Transcribed Spacer regions of ribosomal RNA.

    Fungal diversity

    We used 454 amplicon sequencing to characterize fungal diversity in all pitcher water samples. We prepared multiplex-identifier-tagged PCR libraries from each sample using a primer pair of ITS1F and identifier-tagged ITS4, which amplify the Internal Transcribed Spacer region of fungal ribosomal RNA. We sequenced amplicons at the Duke Genome Sequencing and Analysis Core. Sequence data were processed by trimming barcode and primer sequences; removing all sequences not between 200 and 1000 bp in length; denoising with the QIIME denoiser; and trimming all portions of sequences besides ITS2 (Internal Transcribed Spacer 2) to reduce chimeric sequences. We then clustered sequences into Operational Taxonomic Units (OTUs) with 97% sequence identity and assigned each OTU to a genus using either the NCBI BLAST database or the UNITE Fungal Sequence Database.

    Both alpha and beta fungal diversity were compared among timepoints within the Harvard Forest site and sites. We calculated species richness, standardized using rarefaction for unequal sampling (i.e., number of sequences), for each timepoint and site. We also calculated Bray-Curtis distances among pitchers and timepoints in Harvard Forest and among sites, and visualized these distances using Non-Parametric Multidimensional Scaling (NMDS). We also compared fungal species richness and diversity with presence of C. pseudoglaebosa in samples from Harvard Forest. 454 sequence reads were deposited in NCBI’s Small Reads Archive (SRA), and OTU tables are included as tables here.

    Genotyping C. pseudoglaebosa

    We used Amplified Fragment Length Polymorphisms (AFLP) to genotype C. pseudoglaebosa from different sites in North America. AFLP involves cutting genomic DNA with restriction enzymes, amplifying a subset of fragments, and visualizing amplified fragment sizes using capillary chromatography. Similar fragment size patterns indicate similar genome sequences at and between restriction sites. We used the restriction enzymes EcoRI and MseI, and amplified fragments with four sets of two specific nucleotides next to each restriction site. Fragments were detected using an ABI 3730xls sequencer. All fragments were visually inspected using Genemapper 4.0, fragment sizes were recorded, and fragment presence or absence was determined for each C. pseudoglaebosa isolate. C. pseudoglaebosa genetic distance was calculated from fragment presences and absences, and population structure was inferred from genetic distances. Fragment presence/absence data is included here.

    NCBI Small Reads Archive entries

    Fungal sequences from 2009 S. purpurea collections in Harvard Forest: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA513075

    Fungal sequences from 2010 S. purpurea collections in Harvard Forest and elsewhere: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA821163

  • Organization: Harvard Forest. 324 North Main Street, Petersham, MA 01366, USA. Phone (978) 724-3302. Fax (978) 724-3595.

  • Project: The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. (ROR).

  • Funding: National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210. Other funding: NSF grant DEB-0909694.

  • Use: This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

  • License: Creative Commons Zero v1.0 Universal (CC0-1.0)

  • Citation: Boynton P, Pringle A. 2023. Fungal Diversity in Sarracenia Purpurea Pitchers at Harvard Forest 2009-2010. Harvard Forest Data Archive: HF441 (v.2). Environmental Data Initiative: https://doi.org/10.6073/pasta/a76ded1894ce59ef30f8f6c2028b525b.

Detailed Metadata

hf441-01: operational taxonomic unit table 2009

  1. OTU_ID: operational taxonomic unit
  2. HF116: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  3. HF139: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  4. HF1456: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  5. HF1462: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  6. HF1469: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  7. HF1480: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  8. HF1521: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  9. HF1524: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  10. HF1529: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  11. HF1531: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  12. HF1542: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  13. HF1547: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  14. HF1557: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  15. HF1565: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  16. HF1571: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  17. HF1576: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  18. HF1581: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  19. HF1597: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  20. HF1599: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  21. HF1614: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  22. HF1616: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  23. HF1619: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  24. HF1626: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  25. HF1648: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  26. HF1651: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  27. HF1652: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  28. HF1656: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  29. HF1663: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  30. HF1665: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  31. HF1671: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  32. HF1674: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  33. HF1678: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  34. HF1680: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  35. HF1684: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  36. HF1691: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  37. HF1692: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  38. HF378: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  39. HF380: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  40. HF381: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  41. HF384: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  42. HF393: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  43. HF404: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  44. HF407: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  45. HF414: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  46. HF422: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  47. HF481: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  48. HF498: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  49. HF618: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  50. HF621: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  51. HF627: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  52. HF671: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  53. HF698: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  54. HF706: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  55. ConsensusLineage: assigned taxonomy for each OUT

hf441-02: sample data for 2009 OTU table

  1. Sample: identifier of the pitcher liquid sample
  2. Plant: identifier of the S. purpurea plant from which the sample came
  3. TimePoint: timepoint category of the sample
  4. age: age of the pitcher (days since pitcher opening) at sampling (unit: dimensionless / missing value: NA)
  5. lat: latitude of the plant (unit: dimensionless / missing value: NA)
  6. long: longitude of the plant (unit: dimensionless / missing value: NA)

hf441-03: operational taxonomic unit table 2010

  1. OTU_ID: operational taxonomic unit
  2. BC1: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  3. BC10: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  4. BC2: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  5. BC3: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  6. BC8: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  7. FL11: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  8. FL12: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  9. FL13: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  10. FL15: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  11. FL16: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  12. FL17: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  13. FL18: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  14. GA1: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  15. GA2: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  16. GA3: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  17. GA4: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  18. GA5: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  19. GA6: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  20. GA7: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  21. GA9: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  22. HF1: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  23. HF2: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  24. HF3: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  25. HF5: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  26. HF6: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  27. HF7: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  28. HF9: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  29. NL10: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  30. NL11: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  31. NL12: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  32. NL13: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  33. NL14: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  34. NL3: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  35. NL4: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  36. NL5: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  37. NL6: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  38. NL7: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  39. NL8: number of sequences observed for the OTU in this sample (unit: dimensionless / missing value: NA)
  40. ConsensusLineage: assigned taxonomy for each OUT

hf441-04: sample data for 2010 OTU table

  1. Sample: identifier for the pitcher liquid sample
  2. Location: location from which each sample was taken
  3. date: collection date of the sample
  4. geo_loc_name: geographical location name in NCBI format
  5. lat_lon: latitute and longitude in NCBI format

hf441-05: C. pseudoglaebosa AFLP table

  1. C_pseudoglaebosa_isolate: C. pseudoglaebosa isolate identifiers
  2. AT_CT_1: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  3. AT_CT_2: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  4. AT_CT_3: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  5. AT_CT_4: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  6. AT_CT_5: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  7. AT_CT_6: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  8. AT_CT_7: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  9. AT_CT_8: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  10. AT_CT_9: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  11. AT_CT_10: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  12. AT_CT_11: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  13. AT_CT_12: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  14. AT_CT_13: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  15. AT_CT_14: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  16. AT_CT_15: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  17. AT_CT_16: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  18. AT_CT_17: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  19. AT_CT_18: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  20. AT_CT_19: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  21. AT_CT_20: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  22. AT_CT_21: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  23. AT_CT_22: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  24. AT_CT_23: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  25. AT_CT_24: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  26. AT_CT_25: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  27. AT_CT_26: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  28. AT_CT_27: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  29. AT_CT_28: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  30. AT_CT_29: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  31. AT_CT_30: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  32. AT_CT_31: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  33. AT_CT_32: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  34. AT_CT_33: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  35. CT_TG_1: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  36. CT_TG_2: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  37. CT_TG_3: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  38. CT_TG_4: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  39. CT_TG_5: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  40. CT_TG_6: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  41. CT_TG_7: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  42. CT_TG_8: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  43. CT_TG_9: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  44. CT_TG_10: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  45. CT_TG_11: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  46. CT_TG_12: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  47. CT_TG_13: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  48. CT_TG_14: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  49. CT_TG_15: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  50. CT_TG_16: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  51. TC_CT_1: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  52. TC_CT_2: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  53. TC_CT_3: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  54. TC_CT_4: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  55. TC_CT_5: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  56. TC_CT_6: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  57. TC_CT_7: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  58. TC_CT_8: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  59. TC_CT_9: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  60. TG_CC_1: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  61. TG_CC_2: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  62. TG_CC_3: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  63. TG_CC_4: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  64. TG_CC_5: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  65. TG_CC_6: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  66. TG_CC_7: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  67. TG_CC_8: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  68. TG_CC_9: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  69. TG_CC_10: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  70. TG_CC_11: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  71. TG_CC_12: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  72. TG_CC_13: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence
  73. TG_CC_14: presence or absence of this AFLP peak
    • 1: presence
    • 0: absence

hf441-06: isolate data for C. pseudoglaebosa AFLP table

  1. C_pseudoglaebosa_isolate: C. pseudoglaebosa isolate identifiers from the AFLP table
  2. plant: identifier for the S. purpurea plant from which each C. pseudoglaebosa isolate was isolated
  3. site: location from which each C. pseudoglaebosa isolate was sampled